Church of the Holy Spirit or Holy Spirit Church may refer to:
Estonia
France
India
Germany
- Church of the Holy Spirit, Heidelberg
- Church of the Holy Spirit, Wolfsburg, Germany
- Heilig-Geist-Kirche, Munich
- St. Matthew, Leipzig, formerly Heiliggeistkirche (Church of the Holy Spirit)
- Holy Spirit (Pfaffenhofen an der Ilm)
Italy
- Church of the Holy Spirit (Ravenna)
- Church of the Holy Spirit (Alcamo), Trapani, Sicily
- Church of the Holy Spirit, Palermo, Sicily
Slovakia
Ukraine
- Church of the Holy Spirit, Chernihiv
United Kingdom
- Church of the Holy Spirit, Burpham, Surrey
- Holy Spirit Church, Newtown, Isle of Wight
United States
Other places
- Holy Spirit Church, Bale, Croatia
- Church of the Holy Spirit, Prague, Czech Republic
- Church of the Holy Ghost, Copenhagen, Denmark
- Church of the Holy Spirit (Batumi), Georgia's autonomous republic of Adjara
- Holy Spirit Church (Sajópálfala), Hungary
- Holy Spirit Church Nandakhal, India
- Orthodox Church of the Holy Spirit, Vilnius, Lithuania
- Church of the Holy Spirit, Żejtun, Malta
- Church of the Holy Spirit in Warsaw, Poland
- Church of the Holy Spirit, Lund, Sweden
- Church of the Real Colegio del Espíritu Santo (La Clerecía) in Salamanca; Spain