RMND5B

RMND5B
Identifiers
Aliases	RMND5B, GID2, GID2B, required for meiotic nuclear division 5 homolog B
External IDs	MGI: 1913339; HomoloGene: 100777; GeneCards: RMND5B; OMA:RMND5B - orthologs
Gene location (Human)
Chr.	Chromosome 5 (human)
End	178,150,568 bp
Gene location (Mouse)
Chr.	Chromosome 11 (mouse)
End	51,526,723 bp
RNA expression pattern
	Top expressed in
	gastrocnemius muscle; ; prefrontal cortex; ; epithelium of esophagus; ; anterior pituitary; ; popliteal artery; ; thoracic aorta; ; ascending aorta; ; right adrenal gland; ; body of stomach; ; skin of abdomen;
	Top expressed in
	interventricular septum; ; spermatid; ; hair follicle; ; lip; ; spermatocyte; ; morula; ; seminiferous tubule; ; trigeminal ganglion; ; Region I of hippocampus proper; ; superior frontal gyrus;
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function	ubiquitin-protein transferase activity; protein binding; transferase activity; metal ion binding;
Cellular component	nucleus; cytoplasm; GID complex; cytosol;
Biological process	proteasome-mediated ubiquitin-dependent protein catabolic process; protein ubiquitination; ubiquitin-dependent protein catabolic process;
	Sources:Amigo / QuickGO
Orthologs
	64777
	66089
	ENSG00000145916
	ENSMUSG00000001054
	Q96G75
	Q91YQ7
	NM_001288794; NM_001288795; NM_022762
	NM_025346
	NP_001275723; NP_001275724; NP_073599
	NP_079622
	Wikidata
View/Edit Human	View/Edit Mouse

Required for meiotic nuclear division 5 homolog B (S. cerevisiae), also known as RMND5B, is a protein which in humans is encoded by the RMND5B gene.^[5] It has a zinc finger domain and is highly conserved throughout many eukaryotic organisms.

Protein sequence

This protein is rich in leucine (14.0%) and might belong to the protein family of leucine-rich repeats

        1 meqcacvere ldkvlqkflt ygqhcersle ellhyvgqlr aelasaalqg tplsatlslv       61 msqccrkikd tvqklasdhk dihssvsrvg kaidrnfdse icgvvsdavw dareqqqqil      121 qmaivehlyq qgmlsvaeel cqestlnvdl dfkqpfleln rilealheqd lgpalewavs      181 hrqrllelns slefklhrlh firllaggpa kqlealsyar hfqpfarlhq reiqvmmgsl      241 vylrlgleks pychlldssh waeicetftr dacsllglsv esplsvsfas gcvalpvlmn      301 ikavieqrqc tgvwnhkdel pieielgmkc wyhsvfacpi lrqqtsdsnp piklicghvi      361 srdalnklin ggklkcpycp meqnpadgkr iif

Homology

CAD28476 is highly conserved in many eukaryotic organism. Its high conservation suggests that it plays a primary role in meiosis.

Orthologs

taxonomic name	common name	NCBI entry	Percentage of sequence similarity	Length (AAs)	comments
Homo sapiens	Human	[1]	100%	393	hypothetical Protein for Meiosis
Pan troglodytes	Chimpanzee	[2]	99.7%	393	required for meiotic nuclear division 5 homolog B isoform 6
Macaca mulatta	Rhesus macaque	[3]	98.5%	393	Similar to CG3295-PA isoform 11
Rattus norvegicus	Norway rat	[4]	98.2%	393	Similar to RIKEN cDNA 0610039K22
Mus musculus	House mouse	[5]	97.7%	393	Required for meiotic nuclear division 5 homolog B
Equus caballus	Horse	[6]	97.7%	273	PREDICTED: similar required for meiotic nuclear division 5 homolog B
Bos taurus	Cattle	[7]	95.4%	393	required for meiotic nuclear division 5 homolog B
Danio rerio	Zebrafish	[8]	72.8%	391	required for meiotic nuclear division 5 homolog B
Xenopus laevis	African clawed frog	[9]	70%	391	MGC84431 protein
Canis lupus familiaris	Dog	[10]	70%	391	PREDICTED: similar to CG3295-PA isoform A
Tetraodon nigroviridis	Spotted green pufferfish	[11]	68.5%	417	unnamed protein product
Ornithorhynchus anatinus	Platypus	[12]	64.5%	389
Branchiostoma floridae	Florida lancelet	[13]	57%	491	hypothetical protein BRAFLDRAFT_126901
Nematostella vectensis	Sea anemone	[14]	50.1%	389
Culex quinquefasciatus	Southern house mosquito	[15]	48.5%	392	conserved hypothetical protein
Aedes aegypti	Yellow fever mosquito	[16]	48.2%	392	hypothetical protein AaeL_AAEL009407
Strongylocentrotus purpuratus	Purple urchin	[17]	48.2	405	PREDICTED: Similar to MGC88921 protein
Drosophila virilis	Fruit fly	[18]	41.5%	437	GJ20343
Drosophila melanogaster	Fruit fly	[19]	40.3%	431	CG3295
Acyrthosiphon pisum	Acrythosiphon	[20]	39.5%	366	PREDICTED: required for meiothic nuclear division 5 homolg A
Oryza sativa	Rice	[21]	32.2%	386	membrane protein-like

Zinc finger domain

This depiction shows the location of the two predicted protein domains.

Result of local sequence alignment- the amino acids found in the zinc finger domain are highlighted in different color

Two domains were predicted by the program BLIMPS^[6] to exist in the protein of which one of the domains contains a zinc finger domain.

Zinc finger domains assist the binding of the protein to nucleic acids. This points to a direct interaction of CAD28476 with DNA during meiosis. By comparing CAD28476 with a related zinc finger protein^[7] in a local sequence alignment using LALIGN,^[8] the amino acids His359, Cys381 and Cys384 could be attributed to the zinc finger domain. This zinc finger structure is uncommon in the way that it involves one histidine instead of two.

Expression

Microarray data show that CAD28476 is highly expressed in tissue where meiosis occurs like in testis and ovaries. Moreover, it is also highly expressed in the brain around the hypothalamus.

Transcriptional regulation

The analysis of the promoter region (tools on the page rVista .^[9] were used) shows that there are several transcription factor binding sites localized in conserved regions . It is very likely that the transcription factor Ets-1 which belongs to the ETS transcription factor family and its core binding factor CBF are involved in regulation of transcription since they both have independent binding sites.

Interacting proteins

There were two proteins predicted^[10] which interact with CAD28476.

name of the protein	Uniprot entry	information about the gene
Ewing sarcoma breakpoint region 1, isoform CRA_a	[22]	gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors.
Mothers against decapentaplegic homolog 4	[23]	SMAD4

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